Abstract:
Callus was initiated from hypocotyl and cotyledon explants of
seven of commercial Kazakh cotton cultivars on MS medium
supplemented with 0,1 mg L−1 2,4-D and 0,5 mg L−1 kinetin. The
percentage of callus formation ranged from 64,0 to 91,0% for cotyledons
and from 72 to 90% for hypocotyls. Study of morphological heterogeneity
peculiarities of metamorphosis and histological structure of calli of cotton
allowed to identifying seven tissues types and selecting the
morphologically stable and perspective callus type for embryogenesis.
Significant differences between the calli types of cotton were found by
histological and morphological comparative analysis.
